Analysis Note

ControlWestern blot positive control: U937 cell line

Application

Anti-DAP12 Antibody is an antibody against DAP12 for use in WB.

Research Sub CategoryImmunoglobulins & Immunology

Western blot: 1.0 µg/mL

Optimal working dilutions must be determined by the end user.

Research CategoryInflammation & Immunology

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

Natural killer (NK) cells are lymphocytes that function as a component of innate immunity against intracellular and parasitic pathogens, and possibly tumors. DAP12 is expressed on the surface of NK cells as a disulfide-linked homodimer and contains an immunoreceptor tyrosine based activation motif (ITAM) in its cytoplasmic domain. DAP12 non-covalently associates with members of the killer cell immunoglobulin-like receptor (KIR) family resulting in cellular activation.

Immunogen

Synthetic peptide corresponding to amino acid residues 89-113 of human DAP12 (Accession # AAD09437).

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Physical form

Protein A purified immunoglobulin. Liquid in 0.02M Phosphate Buffer, 0.25M NaCl, with 0.1% sodium azide.

Format: Purified

Specificity

Recognizes human DAP12. This antibody detects a band of approximately 12 kDa in Western blot analysis of U937 cell lysate.

Storage and Stability

Maintain at 2-8°C for up to 12 months from date of receipt.